Soluble DHFR expression concentrations in molecules per cell measured from lysate exercise assays as explained in products and procedures.
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Natural protein sequences are constrained by pressures to maintain required structures and capabilities in just a posh cellular environment. nevertheless, vital cellular factors shaping protein sequences (such as interactions with mobile binding associates or Together with the proteostasis equipment) tend to be unfamiliar. To characterize useful constraints, it's been beneficial to determine mutational landscapes of proteins, which we outline right here as the results on development of every feasible solitary amino acid mutation from the protein, via deep mutational scanning (Boucher et al., 2016; Fowler and Fields, 2014). Deep mutational scanning scientific studies have offered insights into evolution of new protein features (McLaughlin et al.
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